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Specifications
- Antibody Type:Primary
- Antigen Name:Green Fluorescence Protein
- Antigen Symbol:GFP
- Clonality:Monoclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG2a
- Reactivity:
- Western Blot:Yes
- Environmentally Preferable:
- Antigen Synonyms:GFPuv|eGFP|Green Fluorescent Protein
- Storage Buffer:0.02M Potassium phosphate, 0.15M sodium chloride, pH 7.2, containing 0.01% sodium azide
- Storage Temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Immunogen:The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Tested Applications:ELISA, IHC, WB
- Purification:IgG fraction
- Cat. No.:89360-982
- Supplier no.:GTX21218
Specifications
About this item
The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Type: Primary
Antigen: GFP
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Other