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- Antibody Type:Primary
- Antigen Name:Agrin
- Antigen Symbol:AGRN
- Clonality:Monoclonal
- Clone:Agr-131
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1
- Reactivity:Mouse
- Western Blot:Yes
- Size:100 μg
- Environmentally Preferable:
- Gene ID:25592
- Antigen Synonyms:Agrin C-terminal 110 kDa subunit|AGRN|CMS8|Agrin N-terminal 110 kDa subunit|RP23-139J18.2|C90|Agrin C-terminal 90 kDa fragment|AGRIN|Agrin C-terminal 22 kDa fragment|Agrin|CMSPPD|C22
- Storage Buffer:Preservative: NoneConstituents: 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2
- Storage Temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Recombinant rat agrin, C-terminal construct.
- Tested Applications:Radio Immunoassay
- Purification:Protein G purified
- Cat. No.:89359-348
- Supplier no.:GTX12362
Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
ICC: Use at a dilution of 1/50 - 1/1000. IHC-Fr: Use at an assay dependent dilution. RIA: Use at a concentration of 1 ?g/ml. WB: Use at a concentration of 2 ?g/ml. Predicted molecular weight: 208.6 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: AGRN
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Mouse