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- Description:Protein Detector TMB Western Blotting Kit
- Size:25 blots
- Environmentally Preferable:
- Cat. No.:95059-300
- Supplier no.:54-11-50
Protein Detector Western Blot Kits are designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting
Protein Detector TMB Western Blotting Kit contains the most sensitive colorimetric substrate, TMB, a one-component liquid substrate stable for one year. The kit provides protein detection as low as 50 pg. All reagents contained in the kit are ready-to-use and enable convenient and quick assay preparation. Both anti-rabbit and anti-mouse antibodies are supplied for versatility.
The combination of a highly specific, stable liquid conjugate with a sensitive chromogenic substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided. For added flexibility, affinity purified anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase are provided in stabilized, liquid form for quick dilution. TMB Membrane Peroxidase Substrate is provided as a convenient ready-to-use solution, which forms an insoluble blue precipitate when combined with the HRP-labeled antibody.