The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

• Easy to use, high quality and performance
• Higher sensitivity - detect targets down to the low-picogram level
• Longer signal duration - sustained light output for as long as 5 hours
• More imaging options - X-ray, CCD or laser-based imagers

Pierce™ ECL 2 Substrate generates acridinium esters when it reacts with HRP

Pierce™ ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.