Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

• Requires a plate reader or spectrophotometer set to measure at 500 nm
• Proven, well-characterized method for determination of biotinylation levels
• Adaptable to spectrophotometer cuvettes or standard plate readers with 96-well microplates
• Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions
• HABA-avidin complex can be used over a wide range of pH and salt concentrations

HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. This kit contains a premix of HABA and avidin and a biotinylated horseradish peroxidase (HRP) as positive control. The HABA-Avidin premix is supplied in convenient No-Weigh™ microtube packaging, which eliminates the difficulties associated with weighing small quantities of reagent.