Pierce QuantiCleave™ Protease Assay Kits, Thermo Scientific

Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples

No corrosive precipitants used
Entire assay can be run in microplates
Highly sensitive
Faster and uses half the sample of unmodified casein-based protease assays
Measure multiple samples simultaneously in ELISA plate readers
Time/temperature/pH easily manipulated to optimize sensitivity

Each kit can detect proteases as low as 2ng/mL in less than one hour. Entire assays can be run in microplates and measured in ELISA plate readers. The colorimetric kit uses fully succinylated casein as a substrate. When the substrate is hydrolyzed, peptide fragments are released that will react with trinitrobenzene sulfonic acid (TNBSA) to produce a yellow color. The fluorescent kit is based on a FITC-labeled casein and can be used in either FRET or FP modes.

These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.

: Protease assay kit includes Succinylated Casein (supplied as a lyophilized salt-free powder) 5x10 mg, 2,4,6-Trinitrobenzene sulfonic acid (TNBSA) 2 mL, TPCK Trypsin standard (40 BAEE units/mg) 50 mg, BupH borate buffer pack (makes 500 mL) 1 pack. Fluorescent protease assay kit FITC-casein (lyophilized) 2.5 mg, TPCK trypsin 50 mg, BupH borate buffer pack (makes 500 mL) 1 pack.

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