To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Protein Delivery:Yes
- Description:Pierce Pro-Ject™ Protein Transfection Reagent
- Environmentally Preferable:
- Cat. No.:PI89850
- Volume:250 µl
- Supplier no.:89850
This Pierce™ protein transfection reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.
- No need to isolate, clone and transfect gene sequences
- Optimum delivery in three to four hours after incubation
- Delivery complex is non-covalent
- Works with proteins, peptides and fluorescently labeled antibodies
The reagent/protein complexes offer optimum delivery within three to four hours after incubation and the complex is noncovalent so it does not interfere with biological activity
Molecules that have been successfully delivered with the transfection reagent include β-galactosidase, fluorescent-antibody, green fluorescent protein (GFP), high and low molecular weight dextran sulfate, phycoerythrin-BSA, caspase 3, caspase 8 and granzyme B.
It attaches to negatively charged cell surfaces and enters the cell either by directly fusing with the plasma membrane or by enodcytosis and subsequent fusion with the endosome. The transfection reagent may be used to study intercellular signaling, cell cycle regulation, apoptosis, oncogenesis, and transcriptional regulation.