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Specifications
- Description:Mitochondria Isolation Kit for Tissue
- Size:1 kit
- Environmentally Preferable:
- Cat. No.:PI89801
- Application:Intact Mitochondria from Mammalian Tissue
- Supplier no.:89801
- Sufficient for:50 Samples each containing 50-200 mg of Soft or Hard Tissue
Specifications
About this item
The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples.
- Quick and convenient - isolate intact mitochondria in less than 60 minutes
- Versatile - offers two methods of isolation from both soft and hard tissue samples
- Multi-sample format - reagent-based approach enables simultaneous processing of multiple samples
- Optional alternate method - reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes
- Bench-top compatible - both procedures can be performed using a microcentrifuge tube
Both reagent based and Dounce homogenization procedures use differential centrifugation to separate the intact mitochondria using a bench top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for many downstream applications, including 1D and 2D western blotting and protein profiling using mass spectroscopy.