NEXTFLEX™ poly(A) beads 2.0 kits offer a convenient method for obtaining pure, intact mRNA upstream of RNA-sequence library prep with improved mRNA yields, low rRNA contamination and a simple protocol.

• 10 ng - 5 µg total starting RNA
• Magnetic bead-based protocol
• No organic solvents or precipitation step required
• Optimized for use with 10 ng - 5 µg of total RNA as starting material
• Versions are available for automated and manual isolation of the mRNA transcriptome
• Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations

Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples for RNA-sequence library preparation. Removal of rRNA avoids the waste of reagents and bioinformatics resources to sequence and align ~85% of total RNA comprising rRNA (which is generally not a target of interest in NGS sequencing experiments). Revvity’s NEXTFLEX™ poly(A) beads 2.0 kit offers an easy and cost-effective method for removing rRNA in RNA-sequence libraries. This product takes advantage of the tract of adenosine residues present at the 3’ ends of a vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines ('oligo dT') immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.