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Specifications
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:TCP1 theta
- Description:Human TCP1 theta ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma or other biological fluids
- Detection Method:Colorimetric
- Time to Results:1 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:30 - 480 ng/L
- Storage Temperature:4 °C
- Sample Volume:40 μl
- Sensitivity:1.16 ng/L
- Regulatory Status:RUO
- Cat. No.:77214-704
Specifications
About this item
Human TCP1 theta ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TCP1 theta in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Detection range: 30 to 480 ng/L
- Sensitivity: 1.16 ng/L
- Sample type: Serum, plasma or other biological fluids
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human TCP1 theta ELISA kit (A312175) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TCP1 theta in An antibody specific for TCP1 theta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TCP1 theta present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TCP1 theta antibody, which also binds the TCP1 theta present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TCP1 theta antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TCP1 theta antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TCP1 theta captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TCP1 theta in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.