To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Assay duration:One step
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:AMELY
- Description:Human AMELY ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma or other biological fluids
- Detection Method:Colorimetric
- Time to Results:1 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:150 - 2400 ng/L
- Storage Temperature:4 °C
- Sample Volume:40 μl
- Sensitivity:10.16 ng/L
- Regulatory Status:RUO
- Cat. No.:77175-016
Human AMELY ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human AMELY in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Human AMELY ELISA kit (A314047) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human AMELY in serum, plasma or other biological fluids. An antibody specific for AMELY has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the AMELY present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-AMELY antibody, which also binds the AMELY present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-AMELY antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-AMELY antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of AMELY captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of AMELY in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.