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Specifications
- Concentration:6 mg/ml
- Description:Cell counting kit, liquid
- Size:500 ml, 10 μl per well
- Environmentally Preferable:
- Cat. No.:76466-042
- Sensitivity:CCK-8 is the highest sensitive dye for the cell based assay.
- Buffer volume:9% NaCl
- Storage temperature:−4 °C (protect from light)
- No. of tests:500 assays
Specifications
About this item
Cell counting kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt. WST-8 [2- (2- methoxy-4-nitrophenyl)-3- (4-nitrophenyl)-5- (2, 4-disulfophenyl)-2H- tetrazolium, monosodium salt]* produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Cell Counting Kit-8 is a one-bottle solution; no premixing of components is required.
- One bottle, ready to use solution
- No organic solvents or isotopes required
- No harvesting, no washing and no solubilization steps
- No toxicity to cell
- More sensitive than MTT, XTT, MTS or WST-1
Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells.
The cell proliferation assay using CCK-8 correlates well with the [(3)H] thymidine incorporation assay. And the CCK-8 assay can also be substituted for the [(3)H]thymidine incorporation assay. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.