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Specifications
- Antibody Type:Primary
- Antigen Name:GFI1B
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 680
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µl
- Environmentally Preferable:
- Epitope:171-270/330
- Form:Liquid
- Gene ID:8328
- Antigen Synonyms:Potential regulator of CDKN1A|Potential regulator of CDKN1A translocated in CML|GFI 1B|GFI1B_HUMAN|GFI-1B|gfi1b|GFI1B protein|Translocated in CML|Growth factor independent protein 1B|Zinc finger protein Gfi-1b|Growth factor independent 1B protein
- Modification:Unmodified
- Storage Buffer:Aqueous buffered solution containing 0.01 M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
- Storage Temperature:Store at −20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping Temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human GFI1B
- Purification:Purified by Protein A
- Cat. No.:76120-958
- Packaging:Vial
Specifications
About this item
Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
Type: Primary
Antigen: GFI1B
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 680
Public Immunogen Range: 171-270/330
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat