An engineered variant of DNase I, DNase I-XT is a salt-tolerant DNA endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5'-phosphorylated and 3'-hydroxylated ends (1,2).

• Salt-tolerant DNA specific endonuclease
• Degrades double-stranded and single-stranded DNA
• Products are short oligos with 5'-phosphate and 3'-OH
• Degradation of DNA templates from in vitro transcription reactions
• Removal of contaminating genomic DNA from RNA samples

DNase I-XT acts on single and double-stranded DNA, chromatin and the DNA strand of RNA:DNA hybrids. While DNase I (#M0303) is inhibited by salt concentrations >50 mM, DNase I-XT exhibits optimal activity between 50 to 100 mM salt and retains 65% and ~40% activity in 200 and 300 mM salt, respectively. This increased salt tolerance makes DNase I-XT the preferred enzyme for DNA template removal from an in vitro transcription (IVT) reaction. Importantly, DNase I-XT is RNase-free, allowing for the complete removal of DNA from RNA preparations while maintaining RNA integrity.