Agl1 Chemically Competent Agrobacterium, Intact Genomics

About this item

Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) cells allow for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.

High transformation efficiencies
Especially useful for cDNA gDNA library construction

Intact Genomics Agrobacterium tumefaciens AGL1 (AGL-1) cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The AGL1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection.

AGL1 contains the Ti plasmid pTiBO542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.

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