Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using real-time quantitative PCR (RT-qPCR). For research use only.

• Enhanced efficiency, specificity, and sensitivity
• Compatible with all real-time PCR instruments

Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using real-time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains reverse transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity.

In addition, the kit contains CDC recommended primers/probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates. IgScript™ reverse transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.