A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples (with COVID Mini) or up to 2304 samples (with COVID Midi) in batches of up to 96 samples in a single run.

The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopore's rapid chemistry can be deployed, enabling users to rapidly attach a barcode, pool samples, and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.

The protocol delivers robust full genome coverage across a wide range of Ct values. Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow.

The COVID Mini bundle* contains: 1 Midnight Kit (EXP-MRT001), 2 Rapid Barcoding kit 96 and 6 MinION Flow cells (R9.4.1),

The COVID Midi bundle** contains: 1 Midnight Kit (EXP-MRT001), 8 Rapid Barcoding kit 96 and 24 MinION Flow cells (R9.4.1).

* Users wishing to run in multiples of 24 will need to buy 12X Flow Cell Wash Kits.
** Users wishing to run in multiples of 24 or 48 will need to buy 12X or 4X Flow Cell Wash Kits, respectively.