To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Description:Anti-SARS-CoV-2 (B.1.1.7) Neutralizing antibody serologic assay itk (spike trimer)
- Size:96 tests
- Environmentally Preferable:
- Cat. No.:103887-874
- Storage temperature:+2…+8 °C
This kit is developed for qualitative detection or titer measurement of Anti-SARS-CoV-2 (B.1.1.7) neutralizing antibody (Spike Trimer) in human serum.
Multiple variants of SARS-CoV-2 are circulating globally and posting new challenges to human health. As concerns over the potential impacts of the variants grow, people ask if one possesses protective neutralizing antibodies (NAbs) against the variants after receiving the currently available vaccines; and if the NAb therapeutic agents are equally effective to treat the mutant infection as the wild type. To facilitate the mutant-related research, drug trials and vaccine assessments, a high-throughput assay to measure neutralizing antibodies against the mutants is in urgent need.
Your experiment will include five simple steps:
a) All reagents were returned to room temperature (20 to 25 °C) before use.
b) Make series the tested sample and control with ditlution buffer, HRP-SARS-CoV-2 Spike protein diluted with dilution buffer.
c) Add the diluted sample, Control and the HRP-SARS-CoV-2 Spike protein add the plate respectively.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.