Q5U Hot Start High-Fidelity DNA Polymerase, New England Biolabs

About this item

Q5U Hot Start high-fidelity DNA Polymerase is a modified version of Q5 high-fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain.

Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (FFPE)
Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
Generate higher assembly efficiency and improve accuracy in USER cloning
Enable room temperature setup with aptamer-based hot start formulation

Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

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