Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
• Species:
  Human
• Size:
  50 µg
• Environmentally Preferable:
• Tag sequence:
  His Tag (SPR & BLI verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Immobilized Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 0.63 μM as determined in a SPR assay (Biacore T200) (QC tested). Loaded Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (F176), His Tag (SPR & BLI verified) (Cat. No. CDA-H5220) with an affinity constant of 0.51 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P08637-1
• Protein Synonyms:
  FCGR3A|CD16A|FCGR3|FCG3|IGFR3
• Protein/Peptide Name:
  Fc gamma RIIIA/ CD16a
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  23.7 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT
• Cat. No.:
  103815-546