Cleave plasmid DNA and map the relative positions of restriction sites.

• Teach your students about Restriction Enzyme action
• Contains stain for both UV or white-light gel viewing
• Use DNA electrophoresis to create a restriction map
• Complete in approx. 1 hour 45 minutes
• Perishable component refills available

In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.

Additional items needed but not included: DNA Electrophoresis equipment, 5 to 50 µl micropipettes, Water Bath, Transilluminator (UV or White-Light depending on which stain you use), Microwave or Hot plate.