Hands-on simulation of CRISPR-Cas9 to target and treat Cystic Fibrosis.

• Teach your students about new CRISPR technology
• Includes all required reagents
• Use DNA electrophoresis to view reach a diagnosis.
• Activity can be completed in 45 minutes

In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis.

Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

Additional items needed but not included: DNA electrophoresis equipment, white-light box, microwave or hot plate.