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- Description:IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
- Size:20 µl
- Environmentally Preferable:
- Cat. No.:MSPP-4440
- No. of wells:96
- Supplier no.:4440
IncuCyte® Caspase-3/7 green apoptosis assay reagent couples the activated caspase-3/7 recognition motif (DEVD) to NucView™488, a DNA intercalating dye to enable quantification of apoptosis over time.
- Inert
- Requires no wash, mix and read protocol
It is specially formulated for use in the IncuCyte® live-cell analysis system and can be added directly to tissue culture wells. It is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non-fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and green fluorescent staining of nuclear DNA. Kinetic activation of caspase-3/7 can be monitored morphologically using live cell imaging and quantified using the IncuCyte® basic analyzer.