CRISPRcraft™ S.p. Cas9 Nuclease is a purified recombinant wild-type Streptococcus pyogenes Cas9 nuclease produced in E. coli, containing a C-terminal 6xHis tag and a C-terminal NLS to allow efficient transport to the nucleus. It is provided at high concentration (10 mg/ml, 62 µM) to enable efficient RNP delivery and compatibility with multiple delivery methods including lipid-based transfection and electroporation.

• Recombinant wild-type Streptococcus pyogenes Cas9 nuclease, with NLS
• Ideal for efficient RNP-based delivery of Cas9/gRNA complexes
• Extensive quality control to ensure activity and purity

CRISPR-Cas9 gene editing is based on activity of Cas9 nuclease, an RNA-guided endonuclease (RGEN). Cas9 catalyzes sequence-specific cleavage of double-stranded DNA by forming a ribonucleoprotein (RNP) complex with a small (~97 nt) guide RNA that directs Cas9 to the desired locus. This precise DNA cleavage enables a variety of in vivo and in vitro applications including gene editing, CRISPR-mediated DNA detection, sequence enrichment and depletion.