GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium

• Room-Temperature PCR Setup with High-Performance Master Mix
• All-in-one master mix: just add template and primers
• Eliminate nonspecific amplification with a hot start enzyme
• Use the green master mix for direct-to-gel analysis, and the colorless master mix when post-amplification fluorescence or absorbance analysis is required

GoTaq G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium—you only need to add primers and template and go! The GoTaq G2 Hot Start Green Master Mix also contains a gel loading dye to facilitate downstream gel analysis. The GoTaq G2 Hot Start Colorless Master Mix contains no gel loading dye and is used when downstream applications require fluorescence or absorbance readings without purification. GoTaq G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity until the initial denaturation step of the amliication reaction, allowing hot-start PCR. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. GoTaq G2 Hot Start Polymerase exhibits 5' --> 3' exonuclease activity.