A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.

• Detects firefly and Renilla Luciferase Activities in a Single Sample
• Renilla luciferase internal control provides more accurate results
• Assay cells directly in growth medium for both reporters
• Extended half-life of 2 hours for each reporter

The Dual-Glo Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient add-and-read system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo Luciferase Assay System provides high Z'-factors for cell-based, high-throughput screening applications. With the Dual-Glo System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results. The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.