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Specifications
- Antibody Type:Primary
- Antigen Name:topoisomerase (DNA) II alpha 170kDa
- Antigen Symbol:TOP2A
- Clonality:Monoclonal
- Clone:TOP2A/1362
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Environmentally Preferable:
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P11388
- Antigen Synonyms:TOP2A
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76194-726
Specifications
About this item
This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml IF: 1-2 ug/ml Western blot: 1-2 ug/ml IHC (FFPE): 0.5-1 ug/ml The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
Type: Primary
Antigen: TOP2A
Clonality: Monoclonal
Clone: TOP2A/1362
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human