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Specifications
- Antibody Type:Primary
- Antigen Name:ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
- Antigen Symbol:UCHL1
- Clonality:Monoclonal
- Clone:31A3
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1 kappa
- Reactivity:Pig,Human,Bovine,Rat,Mouse
- Western Blot:Yes
- Size:100 ug
- Environmentally Preferable:
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P09936
- Antigen Synonyms:UCHL1
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Native protein from brain was used as the immunogen for this PGP9.5 antibody.
- Purification:Protein G affinity chromatography
- Cat. No.:76195-656
Specifications
About this item
This antibody specifically reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. PGP9.5 antibody has been used to show the protein to be a useful prognostic indicator of colorectal, pancreatic and gall bladder cancer. A mutation in the gene is believed to cause a form of Parkinson's disease.
WB: 0.5-1 ug/ml IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PGP9.5 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: UCHL1
Clonality: Monoclonal
Clone: 31A3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Mouse, Rat, Bovine, Pig