A competitive assay that can be used for quantification of PGD₂.

• Measure PGD₂ in multiple sample types Assay 24 samples in triplicate or 36 samples in duplicate Measure PGD₂ levels down to 350 pg/mL Rapid assay; get results in under 4 hours Midpoint: 50% B/B₀: 800 – 1,500 pg/mL

Prostaglandin D2 (PGD₂) is biosynthesized in the brain by soluble, glutathione-independent lipocalin-type PGD₂ synthase. PGD₂ accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD₂ is also synthesized in mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD₂ which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF_2α and other metabolites. Measurement of the parent eicosanoid PGD₂ is appropriate in cell culture lysates and in CSF, where concentrations of several hundred pg/ml have been measured. Cayman’s PGD₂ Express ELISA Kit is a competitive assay that can be used for quantification of PGD₂ in cell culture lysates and enzymatic reactions. This ELISA offers the convenience of a fast assay with a two hour incubation time and one hour development time, while still achieving an assay range of approximately 117.2 – 15,000 pg/mL and a sensitvity (80% B/B₀) of approximately 350 pg/mL.