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Specifications
- Application:
- Method / Format:Enzymatic extraction
- Prep size:Mini
- Refine target molecule:ssDNA
- Sample type:PCR and other enzymatic reactions
- Target molecule:DNA
- Description:PCR Decontamination Kit
- Size:100 Reactions
- Environmentally Preferable:
- Description:PCR Decontamination Kit
- Cat. No.:76003-302
Specifications
About this item
Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.
- Removes DNA contamination from PCR mastermixes
- Improves target detection by reducing background
- Does not effect PCR sensitivity
- Optimized for PCR and probe based qPCR mixes
For the removal of contaminating DNA from primers and mastermixes prior to PCR.
The PCR decontamination kit uses a dsDNase to remove contaminating DNA from mastermixes. The double-strand specific property allows decontamination in the presence of primers and probe.The dsDNase is irreversibly inactivated by heating to 60°C in the presence of DTT, ensuring that DNA template added to the mastermix after inactivation will not be digested. The mastermix can be used immediately after decontamination to run PCR reactions.
AMPIGENE® Taq polymerases have been treated to remove host DNA and additional clean-up is not necessary. The PCR decontamination kit is recommended for decontamination of primers or competitor’s PCR mixes.