This supermix is is a high-performance real-time PCR supermix formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems.

• Compatible with all commercially available and all Bio-Rad real-time qPCR systems
• Highest data precision allowing cost and time savings
• Consistent performance in fast cycling across a range of reaction conditions, primer concentrations and temperature ranges

The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

The Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions and this polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance. The Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation.

Applications include qPCR/real-time PCR, gene expression analysis, absolute quantification, genotyping (allelic discrimination), mutation detection, pathogen detection, viral detection (load), characterization of genetically modified organisms (GMO) and genetic profiling.