For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism.

• Sensitive and accurate. Linear detection range 27 μg/dl (4.8 μM) to 1000 μg/dl (179 μM) iron in 96-well plate assay
• Simple and high-throughput, the procedure involves addition of a single working reagent and incubation for 40 min
• Can be readily automated as a high-throughput assay for thousands of samples per day
• Improved reagent stability and versatility, the optimized formulation has greatly enhanced reagent and signal stability
• Cuvette or 96-well plate assay
• Method: OD590 nm
• Samples: Biological (serum) and environmental samples

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (like hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy. Simple, direct and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery and environmental monitoring. BioAssay Systems’ iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe²⁺. Fe³⁺ in the sample is reduced to Fe²⁺, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590 nm, is directly proportional to the iron concentration in the sample.