High-fidelity polymerase useful for mutagenesis reactions, 3'-overhang removal and 5'-overhang fill-in reactions.

• Appplications: Labeling in vitro, Flushing Ends
• Synthesizes DNA 5´→3´ from a Primed ssDNA Template
• High fidelity enzyme of choice for applications where misincorporation is a concern
• Flexible polymerase used in a variety of molecular applications; active in many Promega 1X restriction enzyme buffers
• Heat inactivated by heating at 75°C for 10 minutes
• Supplied at a concentration of 5-10u/microl

T4 DNA Polymerase catalyzes the 5'-->3' synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3'-->5' proofreading exonuclease, T4 DNA Polymerase contains no 5'-->3' exonuclease activity. T4 DNA Polymerase can be used to fill 5' protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3' overhangs.