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Specifications
- Description:Beta Galactosidase (β-Gal) Inhibitor Screening Kit (Fluorometric)
- Size:100 assays
- Environmentally Preferable:
- Sample Type:Variable: Inhibitors, small molecules, proteins, etc.
- Shelf Life:12 Months
- Ex/em:480/520 nm
- Cat. No.:75832-884
- Storage temperature:–20 °C
- Supplier no.:K827-100
Specifications
About this item
Kit for screening beta galactosidase Inhibitors.
- Simple, HTP compatible, 30 minutes protocol
- A simple and high-throughput adaptable assay kit
- Used to fluorometrically screen, study and characterize potential inhibitors of beta galactosidase within 30 minutes
Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. β-Gal can also be used as a tool to study protein-protein interaction. In BioVision’s Beta-Galactocidase Inhibitor Screening Kit, β-Gal converts β-Gal substrate to give an intensely fluorescent product. In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase. The assay is simple, high-throughput adaptable and can be performed within 30 minutes.