Rat Neural Stem Cells (RNSC) are isolated from cerebral striatum and mesencephalon of E18 fetal rat brains.

• Each lot functionally characterized
• Cryopreserved at second passage
• Tested negative for mycoplasma, bacteria, yeast, and fungi
• Can be can be cultured and propagated for 1- 2 passages prior to induction of phenotypic differentiation

These cells are maintained in undifferentiated proliferative state by culturing them as free floating neurospheres in serum-free medium optimized with growth factors

Storage: Store cryopreserved vials in liquid nitrogen, growth medium at 4 °C in dark, subculture reagent kit at -20 °C and the reagents at 4 °C upon thawing.

Stem cells are multipotent and can be induced to differentiate into neurons, astrocytes and oligodendrocytes. Neural stem cells are a source of donor tissue for prospective neural cell therapy research given the ability of neurosphere to retain multi-lineage potential and proliferative ability in vitro.