PCR Mycoplasma Test Kit I/C utilizes PCR, which was established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals.

• Detection of many Mollicutes strains
• Provides internal control DNA
• All components come lyophilized in PCR tubes

PCR Mycoplasma Test Kit I/C utilizes PCR, which was established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 5 fg of mycoplasma DNA corresponding to 15-20 mycoplasmas per sample volume. The primer set is specific for a highly conserved 16S-rRNA coding region in the mycoplasma genome. This allows for detection of many Mollicutes strains such as M. orale, M. hyorhinis, M. arginini, M. fermentans, M. salivarium, and M. hominis, which are usually encountered as contaminants in cell cultures. PCR Mycoplasma Test Kit I/C also provides internal control DNA (as negative control or for verifying a successful PCR run) as well as a positive control. All components required for the PCR come lyophilized in PCR tubes included in the kit. Samples can be easily prepared by boiling and centrifuging the cell culture supernatant.