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Specifications
- Description:Endoproteinase Lys-C protease, MS grade
- Size:1 x 20 µg
- Environmentally Preferable:
- Cat. No.:PI90051
- Supplier no.:90051
Specifications
About this item
Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
- Can be used in tandem with trypsin to decrease tryptic missed cleavages thus enhancing digestion
- Better protein characterization results from overlapping peptides with complementary chromatographic, ionisation and fragmentation properties thereby increasing sequence coverage
- Effective enzyme activity under highly denaturing conditions
- C-terminal lysine cleavage specificity of 90% for a complex protein sample
- Stable since it is provided in a lyophilized format
This Lys-C is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes
Applications include improved sequence coverage of protein digests, de novo sequencing, epigenetic studies and in-gel and in-solution digestion of proteins.
Lys-C has high activity and specificity for lysine residues resulting in larger peptides and less sample complexity than trypsin (i.e. fewer peptides). Unlike trypsin, Lys-C can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. These unique Lys-C properties ensure high digestion efficiency when used alone or followed by tryptic digestion.