Pierce™ Strong Ion Exchange Spin columns enable rapid protein fractionation and sample preparation based on charge differences. The matrix has a highly porous structure with pores larger than 3000 nm, providing proteins easy access to the membrane's charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.

• Fast and simple - membrane spin format eliminates column packing
• Centrifugal format enables convenient processing of multiple samples in parallel
• Ideal for sample preparation before 1D or 2D PAGE, pre-fractionation and purification of various proteins
• Mini format: for binding up to 4 mg (50 mL capacity, 48 collection tubes)
• Maxi format: for binding up to 80 mg (20 mL capacity, 16 collection tubes)

Applications:

Pierce Strong Ion Exchange Spin Columns use membrane-adsorption as a chromatographic method to fractionate proteins based on their charge differences. The matrix has a highly porous structure with pores larger than 3000 nm, providing proteins easy access to the membrane's charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.

Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.