Pierce™ Gaussia Luciferase Flash Assay Kit, Thermo Scientific

Supplier: Invitrogen

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Supplier: Invitrogen

Certificates

About this item

The Thermo Scientific Pierce Gaussia luciferase flash assay kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

1000 times greater sensitivity allows utilization of smaller numbers of cells
Highest sensitivity assays result in decreased reagent consumption
Secreted luciferase allows real-time assays and kinetic studies without destroying cells
Assays using secreted luciferases require minimal sample handling
Amenable to automation
Convenient and safe - no radioactivity required

Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity.

Kit may be stored at –80 ˚C, or individually at indicated temperatures.

Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our Gaussia-Dura Luc Vectors.)

The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Delivery: Kit contains Gaussia flash Assay buffer (5 or 50 ml) (store at 4 ˚C), 100X Coelenterazine (50 or 500 µl) (store at –80 ˚C), 2X luciferase cell lysis buffer (6 or 60 ml) (store at room temperature).

Caution: For research use only. Not for use in diagnostic procedures.

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