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Specifications
- Antibody Type:Primary
- Antigen Name:Cleavage Stimulation Factor, 3' pre-RNA, Subunit 3, 64kDa
- Antigen Symbol:CSTF64
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Environmentally Preferable:
- Cross Adsorption:No
- Form:liquid
- Gene ID:23283
- Antigen Synonyms:tau variant|CSTFT_HUMAN.|CSTF 64 kDa subunit|TauCstF64|KIAA0689|CF1 64 kDa subunit|Cleavage stimulation factor 64 kDa subunit
- Storage Buffer:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
- Storage Temperature:Store at –20 °C for 12 months
- Concentration:1 μg/μl
- Shipping Temperature:4°C
- Purification:Purified by Protein A
- Cat. No.:10459-326
- Supplier no.:BS-6995R
Specifications
About this item
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
Recommended Dilutions: Western Blot: 1:100-1000; IHC-P: 1:100-500; IF(IHC-P): 1:50-200
Type: Primary
Antigen: CstF-64
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: