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Anti-DDIT3 Rabbit Polyclonal Antibody (Alexa Fluor® 647)
Anti-DDIT3 Rabbit Polyclonal Antibody (Alexa Fluor® 647)
Catalog # 10302-420
Supplier:  Avantor
Anti-DDIT3 Rabbit Polyclonal Antibody (Alexa Fluor® 647)
Catalog # 10302-420
Supplier:  Avantor
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Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    DNA-damage-inducible transcript 3
  • Antigen Symbol:
    DDIT3
  • Clonality:
    Polyclonal
  • Conjugation:
    Alexa Fluor® 647
  • Host:
    Rabbit
  • ImmunoChemistry:
    Yes
  • Isotype:
    IgG
  • Reactivity:
    Human,
    Rat,
    Mouse
  • Size:
    100 µL
  • Environmentally Preferable:
  • Cross Adsorption:
    No
  • Form:
    liquid
  • Gene ID:
    1649
  • Antigen Synonyms:
    DDIT-3|GADD153|CHOP10|CHOP|CCAAT/enhancer-binding protein homologous protein|C/EBP-homologous protein 10|Growth arrest and DNA damage-inducible protein GADD153|C/EBP-homologous protein|CHOP-10|C/EBP zeta|DNA damage-inducible transcript 3 protein|DDIT3
  • Storage Buffer:
    Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Storage Temperature:
    Store at 4°C for 12 months
  • Concentration:
    1 μg/μl
  • Shipping Temperature:
    4°C
  • Immunogen:
    96-120/168
  • Purification:
    Purified by Protein A
  • Cat. No.:
    10302-420
  • Supplier no.:
    BS-1361R-A647

Specifications

About this item

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF1B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

Recommended Dilutions: IF(IHC-P): 1:50-200

Type: Primary
Antigen: DDIT3
Clonality: Polyclonal
Clone:
Conjugation: Alexa Fluor® 647
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat