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- Antibody Type:Primary
- Antigen Name:splicing Factor SF3B4
- Antigen Symbol:splicing Factor SF3B4
- Clonality:Monoclonal
- Clone:3A1
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b
- Reactivity:Pig,Human,Bovine,Rat,Mouse
- Western Blot:Yes
- Size:100 µg
- Environmentally Preferable:
- Cross Adsorption:No
- Format:Lyophilized
- Antigen Synonyms:SF3B4|SF3b50|SAP49
- Immunogen:Full length recombinant human SF3B4 which was expressed in and purified from E. coli.
- Cat. No.:10782-098
- Supplier no.:M-1576-100
SF3B4 is one of 8 subunits of splicing factor SF3B. SF3B4 is ubiquitously expressed in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells.
Antibody recognizes SF3B. Approved applications include: WB, IHC/IF. Recommended dilution of at least 1:100 for IHC/IF. In WB using chemiluminescence it can be used at dilutions of 1:1,000 or lower. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49kDa. For tissues or cell stain, acetone fixation or light PFA fixation is suggested. The antibody has not yet been tested on paraffin embedded tissues.
Application Information:
WB, ICC, Flow Cytometry. Recommended dilution of 1:500-1:2,000 for ICC. In WB using chemiluminescence it can be used at dilutions of 1:1,000 or lower. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49 kDa. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: splicing factor SF3B4
Clonality: Monoclonal
Clone: 3A1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human, Bovine, Pig, Mouse, Rat