Aminex® Carbohydrate Analysis HPLC Columns separate compounds using a combination of size exclusion and ligand exchange mechanisms.

Size exclusion is the primary mechanism for both monosaccharide and oligosaccharide separations. For monosaccharide separations ligand exchange is affected by the nature of the counterion (Pb²⁺, Ca²⁺, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups. For oligosaccharides low cross-linked resins allow carbohydrates to penetrate, and oligosaccharides to separate by size. Aminex® Columns allow the use of simple isocratic methods, eluting with water or dilute acid. Usually with minimal sample preparation, just filtering through a 0.45 µm filter with no derivatization necessary. Aminex® media have a high pressure stability, wide pH stability, and high column efficiency and selectivity. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3 to 5 minutes. Aminex® HPX-87C Column, for most general sweetener analyses, is optimized for analyzing monosaccharides and also provides class separation of di-, tri-, and tetrasaccharides. The 300×7.8 mm column is used primarily for the quantitation of glucose and fructose in high fructose corn syrup and for general monosaccharide analysis. The 250×4.0 mm column is appropriate for sugar alcohol separations.

Aminex® HPX-87P Column is tailored for the separation of cellulose-derived monosaccharides. This column is used in analyses of pentoses and hexoses in wood products, especially cellobiose, glucose, xylose, galactose, arabinose, and mannose, and also provides excellent resolution of sucrose, lactose, and fructose in dairy products. Fast Carbohydrate Analysis Column is designed for extremely fast separations of specific carbohydrates in samples where only certain components are of interest. The column is optimized for 5-minute analyses of sucrose, glucose, galactose, and fructose. Aminex® HPX-87H Column is engineered for analysis of carbohydrates in solution with carboxylic acids, volatile fatty acids, short chain fatty acids, alcohols, ketones, and many neutral metabolic by-products. Most often used for organic acid analysis, this column is also useful for fermentation monitoring, biological fluid analysis, and acetylated amino sugar separations.

Aminex® HPX-87N Column is optimized for the analysis of sugars in samples with high salt concentrations, such as beet sugars. This column is compatible with salts, so there is no requiement for desalting samples before analysis. Aminex® HPX-87K Column for the analysis of mono-, di-, and trisaccharides in samples such as corn syrup and brewing wort, exhibits good separations of glucose, maltose, and maltotriose. Aminex® HPX-42A Column, for fast, high-resolution oligosaccharide analysis, separates oligosaccharides to D-11 in approximately 25 minutes. Aminex® HPX-42C Column is optimized for analysis of mono- and disaccharides in starch hydrolysates, and provides excellent resolution of oligosaccharides as large as D-10. In-Line Carbohydrate Deashing System protects Aminex® carbohydrate columns from contamination by removing all inorganic salts from the sample, allowing only neutral carbohydrates to pass to the analytical column. The system consists of two Micro-Guard cartridges, one packed with anion and the other with cation exchange resin. Installed in series in front of Bio-Rad analytical columns, this system offers convenient and highly efficient in-line purification of sugar samples.

Aminex® HPX-87H Column is engineered for analysis of carbohydrates in solution with carboxylic acids, volatile fatty acids, short chain fatty acids, alcohols, ketones, and many neutral metabolic by-products. Most often used for organic acid analysis, this column is also useful for fermentation monitoring, biological fluid analysis, and acetylated amino sugar separations.

Aminex® HPX-87N Column is optimized for the analysis of sugars in samples with high salt concentrations, such as beet sugars. This column is compatible with salts, so there is no requiement for desalting samples before analysis. Aminex® HPX-87K Column for the analysis of mono-, di-, and trisaccharides in samples such as corn syrup and brewing wort, exhibits good separations of glucose, maltose, and maltotriose.