Soluble in 50 mg/mL Water. Typically, the effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%.

There is approximately one HCl per lysine unit. The HCl allows it to be a crystalline solid and soluble in water. To remove the HCl, solubilize the product in a neutral buffer and dialyze away the salts.

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates. Poly-L-lysine enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When adsorbed to the culture surface, poly-L-lysine increases the number of positively charged sites available for cell binding. Polymers of both D- and L-lysine are used to coat solid surfaces. Poly-L-lysine has been reported to improve the protein coating of ELISA plates. Other uses for poly-lysine have been reported as follow: 1.Conjugation to methotrexate for increased drug transport. 2.Microencapsulation of islets. 3.Use in simple reproducible procedure for chromosomal preparations from a variety of tissues. 4.Immobilation of living cells in biocompatible semipermeable microcapsules. 5.Poly-L-Lysine In the preparation of polycationic beads. 6.Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.