For separations of proteins and their variants using Thermo Scientific™ ProPac SCX and WCX strong and weak cation-exchange columns, which provide exceptionally high resolution.

• Characterisation and quality control assessment of monoclonal antibodies and other proteins
• Useful for characterisation of related protein variants including deamidation and monoclonal antibodies lysine truncation variants

Based on stationary phases composed of 10 µm nonporous polymeric beads, these columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.