HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.

• High binding capacity – binds up to 40 mg his-tag protein/mL resin
• Excellent flow properties – great performance even at high flow rates
• Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins
• HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems

HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.