About this item
Bio-Rad 0.2 µm pure nitrocellulose membrane is recommended for blotting nucleic acids <550 bp and low molecular weight proteins. The 0.2 µm pore size minimizes protein sample loss due to transfer through the membrane. For larger target proteins and nucleic acids, Bio-Rad 0.45 µm nitrocellulose is recommended.
Applications:
Precut membrane/blot absorbent filter paper sandwiches, precut membrane sheets and membrane rolls are available in a range of sizes.
* Transfer of low molecular–weight proteins and nucleic acids <500 bp
* Protein transfer from SDS-PAGE gels to membrane in preparation for western blotting
* Transfer of nucleic acids in preparation for northern or Southern blotting
* Immunodetection of target protein and probe hybridization of nucleic acids
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