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Specifications
- Description:TSS (Transformation and Storage Solution)
- Size:125 ml
- Environmentally Preferable:
- Cat. No.:AAJ62318-EQE
- pH:6.5
- Storage temperature:RT
- Supplier no.:J62318-EQE
Specifications
About this item
TSS (transformation and storage solution) buffers can be used to make chemically competent cells.
- Contains 85% LB medium, 10% (w/v) PEG MW 8000, 5% (v/v) DMSO, 50 mM magnesium chloride
- Fully miscible in water
- Sterile filtered
TSS solution is used in the one-step procedure for the preparation of competent E. coli and allows transformation of cells without heat-shock. Transformation efficiencies of 106 to 108/µg DNA are typically obtained using TSS.