You searched for: Western Blotting Reagents

Supplier: Invitrogen

Pierce Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.

Supplier: Surmodics

BioFX® Chemiluminescent Ultra Sensitive HRP Membrane Substrate is a luminol based formulation for western blot applications.

Supplier: Invitrogen

The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Supplier: Alkali Scientific

Enhance your Western blotting experiments with the BioBlue-Lite™ Western Blot Film, ensuring reliable and high-quality results for your research.

Supplier: Invitrogen

Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Supplier: Invitrogen

The Pierce™ Far-Western Blot Kit for Biotinylated Proteins provide a nonisotopic approach to far-Western analysis that can be performed either directly in the gel or on the membrane

Supplier: ADVANSTA INC MS

Advansta's Quantum is ideal for quantitative chemiluminescent Western blotting.

Supplier: ADVANSTA INC MS

Advansta's HRP substrate is the best choice for routine, film-imaged chemiluminescent Western blots.

Supplier: Invitrogen

The 1-Step™ TMB-blotting solution is a blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blot and IHC experiments.

Supplier: Invitrogen

The Thermo Scientific SuperSignal West HisProbe Kit contains HisProbe-HRP Conjugate, as well as reagents required for detection of polyhistidine-tagged protein by Western blotting

Supplier: Invitrogen

Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

Supplier: Invitrogen

An advanced formula for removing high-affinity bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Supplier: Surmodics

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers.

Supplier: Surmodics

BioFX® Chemiluminescent Super Sensitive Plus HRP Substrate provides a higher degree of sensitivity in the detection of peroxidase than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays.

Supplier: Invitrogen

Thermo Scientific HisProbe-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

Supplier: Invitrogen

The Thermo Scientific Pierce DAB Substrate Kit enables chromogenic detection of horseradish peroxidase (HRP) activity based the action of 3,3'-diaminobenzidine (DAB) in Western blot and tissue staining methods.

Supplier: Surmodics

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and Western Blot assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the luminol substrate, antibody/antigen titers and blockers. Surmodics provides multiple 2-component HRP chemiluminescent substrates with various intensities to allow the assay developer to choose the appropropriate substrate for the application.

Supplier: Invitrogen

LightShift® Chemiluminescent EMSA Kit is robust and sensitive system for performing electrophoretic mobility shift assays (EMSAs) to identify and characterize protein:DNA binding interactions.

Supplier: VWR

VisiGlo HRP and VisiGlo Plus HRP are luminol-based chemiluminescent substrates designed for the rapid and sensitive detection of peroxidase-labeled conjugates.

Supplier: Azure Biosystems

Radiance Q chemiluminescent substrate sets the bar for sensitivity and quantitative ability

Supplier: Azure Biosystems

Azure’s proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3.5, Cy5, Cy5.5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: Enzo Life Sciences

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