Anti-CDC27 Rabbit Polyclonal Antibody

Catalog # 10800-062

Catalog # 10800-062

Supplier Number: 600-401-Y33

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Specifications

Antibody Type:
Primary
Antigen Name:
Cell Division Cycle 27
Antigen Symbol:
CDC27
Clonality:
Polyclonal
Conjugation:
Unconjugated
ELISA:
Yes
Host:
Rabbit
ImmunoChemistry:
Yes
Isotype:
Reactivity:
Human,
Rat,
Mouse
Western Blot:
Yes
Size:
100 µg
Environmentally Preferable:
Epitope:
C-Terminal
Format:
IgG
Form:
Liquid (sterile filtered)
Gene ID:
996
Antigen Synonyms:
CDC27Hs|H-NUC|APC3|HNUC|D17S978E|D0S1430E|ANAPC3|NUC2
Concentration:
1 mg/mL by UV absorbance at 280 nm
Shipping Temperature:
Dry Ice
Immunogen:
Anti-APC3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 19 amino acid synthetic peptide near the C-terminus of human APC3.
Purification:
Anti-APC3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with APC3 from other sources has not been determined.
Cat. No.:
10800-062
Supplier no.:
600-401-Y33

Specifications

About this item

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.

Recommended Dilutions: ELISA: 1:10,000-1:20,000; Immunohistochemsitry: 5 ?g/mL; Western Blot: 1 - 2 µg/mL; contains 0.02% (w/v) Sodium Azide

Type: Primary
Antigen: CDC27
Clonality: Polyclonal
Clone: 0
Conjugation: Unconjugated
Epitope: C-Terminal
Host: Rabbit
Isotype:
Reactivity: